Measurement
Part:BBa_K4204016:Design
Designed by: Jianfei Song Group: iGEM22_BNDS_China (2022-10-08)
Crystal violet induced sfGFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 907
Illegal PstI site found at 456
Illegal PstI site found at 792 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 907
Illegal NheI site found at 1005
Illegal PstI site found at 456
Illegal PstI site found at 792 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 907
Illegal BglII site found at 916
Illegal XhoI site found at 1728 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 907
Illegal PstI site found at 456
Illegal PstI site found at 792 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 907
Illegal PstI site found at 456
Illegal PstI site found at 792
Illegal NgoMIV site found at 477
Illegal AgeI site found at 327
Illegal AgeI site found at 1158 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There must not have any gap between the RBS and the start codon in this circuit. Gaps may cause lower transcriptional activity, or even shift of open reading frame.
Source
The crystal violet induction system is from Ruegg et al.'s work [1]. Assembled by BNDS_China 2022.
References
[1] Ruegg, T. L., Pereira, J. H., Chen, J. C., DeGiovanni, A., Novichkov, P., Mutalik, V. K., Tomaleri, G. P., Singer, S. W., Hillson, N. J., Simmons, B. A., Adams, P. D., & Thelen, M. P. (2018). Jungle Express is a versatile repressor system for tight transcriptional control. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05857-3